Immobilon-FL PVDF转印膜

32031602

NB.22

The Immobilon^®-FL transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 μm. It is designed for binding proteins transferred from a variety of gel matrices. The Immobilon^®-FL transfer membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane offers optimal blotting for proteins with molecular weights greater than 20,000. The Immobilon^®-FL membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. Immobilon^®-FL membrane can also be used for standard chemiluminescent or chromogenic detection.

Immobilon-FL PVDF Membrane has been used in western blotting.

Immobilon-FL Membrane:

• The first transfer membrane optimized for fluorescence applications; Extremely low background enhances the sensitivity of all fluorescence detection protocols
• Compatible with all commonly used fluorescent probes at a wide range of excitation/emission wavelengths
• Best for multiplexing and chemifluorescence detections
• compatible with standard blocking agents and buffers

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

PVDF membrane
plain filter
white filter

400

hydrophobic

Immobilon^®

western blot: suitable

26.5 cm × 3.75 m

0.45 μm pore size

155 μg/cm² adsorption capacity (insulin)
205 μg/cm² adsorption capacity (BSA)
300 μg/cm² adsorption capacity (goat IgG)

for use with All fluorescence dyes
for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

chemiluminescent
colorimetric
fluorometric

ambient

11 - Combustible Solids

WGK 3