pIEx-2 DNA-Novagen

100

Novagen^®

OK to freeze

−20℃

The pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-2 contains N-terminal GST•Tag, His•Tag, and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
pIEx-2 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

For sequencing or PCR of recombinants in pIEx-2, refer to the table below to choose the appropriate primers.

Toxicity: Standard Handling (A)

INSECTDIRECT is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

10 - Combustible liquids

WGK 1