MOPS-EDTA-乙酸钠 (MESA)

200

for molecular biology

BioReagent

powder

6.8 - 7.2 (reconstituted per directions)

H₂O: soluble, faintly yellow

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

room temp

1 L in poly bottle

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

1x MESA buffer contains:

• 40 mM MOPS
• 10 mM sodium acetate
• 1 mM EDTA (pH 8.3)

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

11 - Combustible Solids

WGK 3

Eyeshields, Gloves, type N95 (US)